Spectrophotometric Determination of Uric Acid in Samples of Urine

Authors

  • Abbas. H. Al-Shukry College of Health and Medical Technologies, Uruk University, Baghdad, Iraq.
  • Zain E. Mansor College of Health and Medical Technologies, Uruk University, Baghdad, Iraq.
  • Nada. A. Abd Al -Hussein College of Health and Medical Technologies, Uruk University, Baghdad, Iraq.College of Health and Medical Technologies, Uruk University, Baghdad, Iraq.

A fade of the hexadecyltrimethylammonium-o-hydroxyhydroquinonephthalein-palladium (II) complex was used to quantify uric acid (UA), a spectrophotometric technique that is both straightforward and very sensitive. The effective molar absorbance at 635 nm complies with Beer's law over a range of concentration of 0.01 g ml-1, with a relative standard deviation of 6.50 × 105 dm3/mol.cm (˂1.5%).  Overall, UA was recovered in the range of 98-100% across all samples, with values were of relative standard deviation (RSD) < 3.0%.  This review paper is based on the elaborate work [1], giving rise to very accurate for uric acid assay using UV-VIS spectrometry system, which is about 20 times more sensitive than other methods reported elsewhere. Human urine uric acid assays using this technique were of high accuracy.

Keywords:

Uric acid, , UV- spectrometry, Atomic absorption, UA- analysis, UA-determination, extraction

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Spectrophotometric Determination of Uric Acid in Samples of Urine . (2024). Journal Port Science Research, 7(1), 22-29. https://doi.org/10.36371/port.2024.1.4

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Spectrophotometric Determination of Uric Acid in Samples of Urine . (2024). Journal Port Science Research, 7(1), 22-29. https://doi.org/10.36371/port.2024.1.4

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